Embryos requireculture media in the laboratory to optimise their development by supporting metabolic and physiological phases before implantation. Improvement of embryo culture has contributed significantly to the increase in the overall success rates in assisted reproductive technology (ART).

EmbryoGen® and BlastGen™ are new sequential culture media. Fertilised and cleavage-stage embryos (zygote to day three) are cultured with EmbryoGen®, followed by the culture to the blastocyst stage with BlastGen™. For the embryos cultured with this system, BlastGen™ is the recommended medium for embryo transfer.

EmbryoGen® and BlastGen™ are suitable for all patients and are especially recommended for people who have experienced recurrent biochemical pregnancy loss, miscarriage, recurrent implantation failures or unexplained infertility. EmbryoGen® and BlastGen™ are culture media that have been developed with the addition of the cytokine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). GM-CSF is a protein expressed in the female reproductive tract, and is upregulated throughout embryo development. Research has shown that an addition of 2ng/ml of GM-CSF to culture media improved embryo growth and development.

GM-CSF appears to assist embryo development in several ways:

  • Regulates cell survival, proliferation and differentiation.
  • Regulates immune response.
  • Enhances cell signalling.
  • Benefits cell-to-cell communication.
  • Promotes blastocyst formation.
  • Pre-implantation embryos express receptors for GM-CSF.

Helps in the regulation of implantation.

EmbryoGen® and BlastGen™ work to reduce the embryo stress by providing a more physiological and natural in vitro environment. They improve the communication between the mother and embryo and offer a low cost, non-invasive alternative rather than undertaking harsher, more expensive treatments for failed cycles or miscarriages.

A large clinical trial conducted in Denmark and Sweden observed the effects of GM-CSF and its addition in culture media of patients undergoing ART. Studies have shown a significant increase in survival of transferred embryos and lower miscarriage rates. GM-CSF displays positive effects on embryo development.

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